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To date, the exact binding site of MIF to CD74 isn't crystal clear, but compact molecules and mutations targeting the tautomerase active web site pocket are shown to interfere Along with the binding of MIF to CD74 [forty four]. Curiously, enthusiast et al. manufactured just one cysteine mutant of MIF (MIFN110C) that covalently locks MIF right into
The amino-terminal proline residue is very important to the catalytic action; having said that, it is unclear no matter whether a purposeful enzyme exercise of MIF is needed for its biological function33,34,35,36. It is possible the enzyme functions of MIF signify vestigial signatures with the prevalent ancestral origin of MIF and DDT genes. howeve